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X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) MUTATION W48F, F200T

Released:
DOI: 10.2210/pdb1ldf/pdb
PDB entry 1ldf coloured by chain, front view.
PDB entry 1ldf coloured by chain, side view.
PDB entry 1ldf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Control of the selectivity of the aquaporin water channel family by global orientational tuning.
Tajkhorshid E, Nollert P, Jensen MØ, Miercke LJ, O'Connell J, Stroud RM, Schulten K
Science 296 525-30 (2002)
PMID: 11964478

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol uptake facilitator protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 29.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AER0 (Residues: 1-281; Coverage: 100%)
Gene names: JW3898, b3927, glpF
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

3 bound ligands:
Ligand BOG 2 x BOG
Ligand MG 2 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: I422
Unit cell:
a: 96.925Å b: 96.925Å c: 185.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.243
Expression system: Escherichia coli K-12

Quick links

Citations

95 review citations

CHARMM: the biomolecular simulation program.
Brooks et al. (2009)
94 more

16 mentions without citation

Life at the border: adaptation of proteins to anisotropic membrane environment.
Pogozheva et al. (2014)
15 more