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X-ray diffraction
1.74Å resolution

PLASMODIUM FALCIPARUM L-LACTATE DEHYDROGENASE COMPLEXED WITH NADH AND OXAMATE

Released:
DOI: 10.2210/pdb1ldg/pdb
PDB entry 1ldg coloured by chain, front view.
PDB entry 1ldg coloured by chain, side view.
PDB entry 1ldg coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design.
Dunn CR, Banfield MJ, Barker JJ, Higham CW, Moreton KM, Turgut-Balik D, Brady RL, Holbrook JJ
Nat Struct Biol 3 912-5 (1996)
PMID: 8901865

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.16 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27743 (Residues: 1-316; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 1 x NAI
1 bound ligand:
Ligand OXM 1 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: I222
Unit cell:
a: 80.2Å b: 86.8Å c: 90.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.235
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Rapid diagnostic tests for malaria parasites.
Moody A. (2002)
11 more

20 mentions without citation

Metabolic Diversity and Therapeutic Potential of Holarrhena pubescens: An Important Ethnomedicinal Plant.
Zahara et al. (2020)
19 more