› 1ldm

X-ray diffraction
2.1Å resolution

Refined crystal structure of dogfish M4 apo-lactate dehydrogenase

Released:
DOI: 10.2210/pdb1ldm/pdb
PDB entry 1ldm coloured by chain, front view.
PDB entry 1ldm coloured by chain, side view.
PDB entry 1ldm coloured by chain, top view.
Source organism: Squalus acanthias
Primary publication:
Refined crystal structure of dogfish M4 apo-lactate dehydrogenase.
Abad-Zapatero C, Griffith JP, Sussman JL, Rossmann MG
J Mol Biol 198 445-67 (1987)
PMID: 3430615

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 36.33 KDa
Source organism: Squalus acanthias
Expression system: Not provided
UniProt:
  • Canonical: P00341 (Residues: 2-333; Coverage: 99%)
Gene name: ldha
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
Ligand OXM 2 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
Unit cell:
a: 134.5Å b: 134.5Å c: 85.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

Review: Protein function at thermal extremes: balancing stability and flexibility.
Fields PA. (2001)
8 more

16 mentions without citation

The Lactate and the Lactate Dehydrogenase in Inflammatory Diseases and Major Risk Factors in COVID-19 Patients.
Gupta GS. (2022)
15 more