› 1lem

X-ray diffraction
3Å resolution

THE MONOSACCHARIDE BINDING SITE OF LENTIL LECTIN: AN X-RAY AND MOLECULAR MODELLING STUDY

Released:
DOI: 10.2210/pdb1lem/pdb
PDB entry 1lem coloured by chain, front view.
PDB entry 1lem coloured by chain, side view.
PDB entry 1lem coloured by chain, top view.
Source organism: Lens culinaris
Primary publication:
The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study.
Loris R, Casset F, Bouckaert J, Pletinckx J, Dao-Thi MH, Poortmans F, Imberty A, Perez S, Wyns L
Glycoconj J 11 507-17 (1994)
PMID: 7696853

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136278 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lectin beta chain Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 19.91 KDa
Source organism: Lens culinaris
Expression system: Not provided
UniProt:
  • Canonical: P02870 (Residues: 31-211; Coverage: 74%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls
Lectin alpha chain Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 5.71 KDa
Source organism: Lens culinaris
Expression system: Not provided
UniProt:
  • Canonical: P02870 (Residues: 218-269; Coverage: 21%)

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand CA 1 x CA
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6522
Unit cell:
a: 85.71Å b: 85.71Å c: 165.38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 not available
Expression system: Not provided

Quick links

Citations

5 review citations

Plant lectins: the ties that bind in root symbiosis and plant defense.
De Hoff et al. (2009)
4 more

1 mention without citation

Energetics of galactose- and glucose-aromatic amino acid interactions: implications for binding in galactose-specific proteins.
Sujatha et al. (2004)