› 1lf6

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE

Released:
DOI: 10.2210/pdb1lf6/pdb
PDB entry 1lf6 coloured by chain, front view.
PDB entry 1lf6 coloured by chain, side view.
PDB entry 1lf6 coloured by chain, top view.
Source organism: Thermoanaerobacterium thermosaccharolyticum
Primary publication:
Crystal structure and evolution of a prokaryotic glucoamylase.
Aleshin AE, Feng PH, Honzatko RB, Reilly PJ
J Mol Biol 327 61-73 (2003)
PMID: 12614608

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucoamylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 684 amino acids
Theoretical weight: 76.59 KDa
Source organism: Thermoanaerobacterium thermosaccharolyticum
UniProt:
  • Canonical: O85672 (Residues: 18-141, 142-695; Coverage: 100%)
Gene name: ga1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 81.186Å b: 102.513Å c: 164.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.232

Quick links

Citations

5 review citations

Microbial glucoamylases: characteristics and applications.
Kumar et al. (2009)
4 more

4 mentions without citation

Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron.
Kitamura et al. (2008)
3 more