› 1lgc

X-ray diffraction
2.8Å resolution

INTERACTION OF A LEGUME LECTIN WITH THE N2 FRAGMENT OF HUMAN LACTOTRANSFERRIN OR WITH THE ISOLATED BIANTENNARY GLYCOPEPTIDE: ROLE OF THE FUCOSE MOIETY

Released:
DOI: 10.2210/pdb1lgc/pdb
PDB entry 1lgc coloured by chain, front view.
PDB entry 1lgc coloured by chain, side view.
PDB entry 1lgc coloured by chain, top view.
Source organism: Lathyrus ochrus
Primary publication:
Structures of a legume lectin complexed with the human lactotransferrin N2 fragment, and with an isolated biantennary glycopeptide: role of the fucose moiety.
Bourne Y, Mazurier J, Legrand D, Rougé P, Montreuil J, Spik G, Cambillau C
Structure 2 209-19 (1994)
PMID: 8069634

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-137412 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Lectin beta-1 and beta-2 chains Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 181 amino acids
Theoretical weight: 19.86 KDa
Source organism: Lathyrus ochrus
Expression system: Not provided
UniProt:
  • Canonical: P04122 (Residues: 1-181; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls
DIPEPTIDE Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 2 amino acids
Theoretical weight: 260 Da
Mannose/glucose-specific lectin alpha 2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 53 amino acids
Theoretical weight: 5.93 KDa
Source organism: Lathyrus ochrus
Expression system: Not provided
UniProt:
  • Canonical: P12307 (Residues: 1-53; Coverage: 100%)
Sequence domains: Legume lectin domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC, GAL, SIA
Carbohydrate polymer
3 bound ligands:
Ligand CA 3 x CA
Ligand MN 3 x MN
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 117Å b: 117Å c: 120.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 not available
Expression system: Not provided

Quick links

Citations

8 review citations

The structural role of sugars in glycoproteins.
Wyss et al. (1996)
7 more

4 mentions without citation

Legume Lectins with Different Specificities as Potential Glycan Probes for Pathogenic Enveloped Viruses.
Barre et al. (2022)
3 more