› 1lk5

X-ray diffraction
1.75Å resolution

Structure of the D-Ribose-5-Phosphate Isomerase from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb1lk5/pdb
PDB entry 1lk5 coloured by chain, front view.
PDB entry 1lk5 coloured by chain, side view.
PDB entry 1lk5 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
A hyperthermostable D-ribose-5-phosphate isomerase from Pyrococcus horikoshii characterization and three-dimensional structure.
Ishikawa K, Matsui I, Payan F, Cambillau C, Ishida H, Kawarabayasi Y, Kikuchi H, Roussel A
Structure 10 877-86 (2002)
PMID: 12057201

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 229 amino acids
Theoretical weight: 25.19 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50083 (Residues: 1-229; Coverage: 100%)
Gene names: PH1375, rpiA
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 10 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 73.548Å b: 114.932Å c: 119.017Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
2 more

6 mentions without citation

Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate.
Meloni et al. (2023)
5 more