› 1llm

X-ray diffraction
1.5Å resolution

Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA

Released:
DOI: 10.2210/pdb1llm/pdb
PDB entry 1llm coloured by chain, front view.
PDB entry 1llm coloured by chain, side view.
PDB entry 1llm coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a designed dimeric zinc finger protein bound to DNA.
Wolfe SA, Grant RA, Pabo CO
Biochemistry 42 13401-9 (2003)
PMID: 14621985

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114141 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Early growth response protein 1; General control transcription factor GCN4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 88 amino acids
Theoretical weight: 10.55 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P08046 (Residues: 363-412, 415-415; Coverage: 10%)
  • Canonical: P03069 (Residues: 245-246, 248-249, 250-281; Coverage: 13%)
Gene names: AAS101, AAS3, ARG9, Egr-1, Egr1, GCN4, Krox-24, YEL009C
Sequence domains:
Structure domains: Classic Zinc Finger
5'-D(*TP*CP*CP*CP*AP*CP*GP*CP*GP*TP*GP*GP*G)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 13 nucleotides
Theoretical weight: 3.97 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, NSLS BEAMLINE X4A
Spacegroup: P3121
Unit cell:
a: 87.398Å b: 87.398Å c: 118.693Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.216 0.234
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Foundations for engineering biology.
Endy D. (2005)
6 more

12 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
11 more