› 1lls

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON

Released:
DOI: 10.2210/pdb1lls/pdb
PDB entry 1lls coloured by chain, front view.
PDB entry 1lls coloured by chain, side view.
PDB entry 1lls coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy.
Rubin SM, Lee SY, Ruiz EJ, Pines A, Wemmer DE
J Mol Biol 322 425-40 (2002)
PMID: 12217701

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 40.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand XE 1 x XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 38.207Å b: 44.026Å c: 57.649Å
α: 100.75° β: 101.36° γ: 102.62°
R-values:
R R work R free
0.205 0.204 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Crystal structures of fusion proteins with large-affinity tags.
Smyth et al. (2003)
7 more

11 mentions without citation

Secondary structure of Huntingtin amino-terminal region.
Kim et al. (2009)
10 more