› 1lm3

X-ray diffraction
2.7Å resolution

A Multi-generation Analysis of Cytochrome b562 Redox Variants: Evolutionary Strategies for Modulating Redox Potential Revealed Using a Library Approach

Released:
DOI: 10.2210/pdb1lm3/pdb
PDB entry 1lm3 coloured by chain, front view.
PDB entry 1lm3 coloured by chain, side view.
PDB entry 1lm3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A multigeneration analysis of cytochrome b(562) redox variants: evolutionary strategies for modulating redox potential revealed using a library approach.
Springs SL, Bass SE, Bowman G, Nodelman I, Schutt CE, McLendon GL
Biochemistry 41 4321-8 (2002)
PMID: 11914078

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 42.098Å b: 48.995Å c: 114.419Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.333
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
5 more