› 1ln0

X-ray diffraction
2Å resolution

Structure of the Catalytic Domain of Homing Endonuclease I-TevI

Released:
DOI: 10.2210/pdb1ln0/pdb
PDB entry 1ln0 coloured by chain, front view.
PDB entry 1ln0 coloured by chain, side view.
PDB entry 1ln0 coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI.
Van Roey P, Meehan L, Kowalski JC, Belfort M, Derbyshire V
Nat Struct Biol 9 806-11 (2002)
PMID: 12379841

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Intron-associated endonuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 11.38 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13299 (Residues: 1-97; Coverage: 40%)
Gene name: ITEVIR
Sequence domains: GIY-YIG catalytic domain
Structure domains: GIY-YIG endonuclease

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 123.87Å b: 56.58Å c: 35.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.255
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
6 more

8 mentions without citation

Phylogenomic analysis of the GIY-YIG nuclease superfamily.
Dunin-Horkawicz et al. (2006)
7 more