› 1lnw

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE MEXR REPRESSOR OF THE MEXAB-OPRM MULTIDRUG EFFLUX OPERON OF PSEUDOMONAS AERUGINOSA

Released:
DOI: 10.2210/pdb1lnw/pdb
PDB entry 1lnw coloured by chain, front view.
PDB entry 1lnw coloured by chain, side view.
PDB entry 1lnw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the MexR repressor of the mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa.
Lim D, Poole K, Strynadka NC
J. Biol. Chem. 277 29253-9 (2002)
PMID: 12034710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-156449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance operon repressor Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 147 amino acids
Theoretical weight: 17.22 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P52003 (Residues: 1-147; Coverage: 100%)
Gene names: PA0424, mexR
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 67.91Å b: 72.654Å c: 240.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.294
Expression system: Escherichia coli

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Citations

15 review citations

Efflux-mediated drug resistance in bacteria.
Li et al. (2004)
14 more

16 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
15 more