› 1lom

X-ray diffraction
1.72Å resolution

CYANOVIRIN-N DOUBLE MUTANT P51S S52P

Released:
DOI: 10.2210/pdb1lom/pdb
PDB entry 1lom coloured by chain, front view.
PDB entry 1lom coloured by chain, side view.
PDB entry 1lom coloured by chain, top view.
Source organism: Nostoc ellipsosporum
Primary publication:
Domain-swapped structure of a mutant of cyanovirin-N.
Botos I, Mori T, Cartner LK, Boyd MR, Wlodawer A
Biochem Biophys Res Commun 294 184-90 (2002)
PMID: 12054761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanovirin-N Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.02 KDa
Source organism: Nostoc ellipsosporum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P81180 (Residues: 1-101; Coverage: 100%)
Sequence domains: CVNH domain
Structure domains: Cyanovirin-N

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 48.093Å b: 48.093Å c: 79.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.267 0.267 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Proteins that bind high-mannose sugars of the HIV envelope.
Botos et al. (2005)
11 other articles

3 mentions without citation

Anti-HIV activity of defective cyanovirin-N mutants is restored by dimerization.
Matei et al. (2010)
2 more