› 1lrz

X-ray diffraction
2.1Å resolution

x-ray crystal structure of staphylococcus aureus femA

Released:
DOI: 10.2210/pdb1lrz/pdb
PDB entry 1lrz coloured by chain, front view.
PDB entry 1lrz coloured by chain, side view.
PDB entry 1lrz coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
X-ray crystal structure of Staphylococcus aureus FemA.
Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL
Structure 10 1107-15 (2002)
PMID: 12176388

Function and Biology Details

Reaction catalysed: 
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N(6)-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNA = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N(6)-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoacyltransferase FemA Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 50.03 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0A5 (Residues: 1-420; Coverage: 100%)
Gene name: femA
Sequence domains: FemAB family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 53.93Å b: 90.36Å c: 109.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.212
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structure and functions of the GNAT superfamily of acetyltransferases.
Vetting et al. (2005)
13 more

11 mentions without citation

Bacterial GCN5-Related N-Acetyltransferases: From Resistance to Regulation.
Favrot et al. (2016)
10 more