› 1lvl

X-ray diffraction
2.45Å resolution

THE REFINED STRUCTURE OF PSEUDOMONAS PUTIDA LIPOAMIDE DEHYDROGENASE COMPLEXED WITH NAD+ AT 2.45 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1lvl/pdb
PDB entry 1lvl coloured by chain, front view.
PDB entry 1lvl coloured by chain, side view.
PDB entry 1lvl coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
The refined crystal structure of Pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 A resolution.
Mattevi A, Obmolova G, Sokatch JR, Betzel C, Hol WG
Proteins 13 336-51 (1992)
PMID: 1325638

Function and Biology Details

Reaction catalysed: 
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyl dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 48.08 KDa
Source organism: Pseudomonas putida
Expression system: Not provided
UniProt:
  • Canonical: P09063 (Residues: 2-459; Coverage: 100%)
Gene name: lpdV
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 64.32Å b: 108.12Å c: 151.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 not available not available
Expression system: Not provided

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Citations

2 review citations

The pyruvate dehydrogenase multi-enzyme complex from Gram-negative bacteria.
de Kok et al. (1998)
1 more

12 mentions without citation

A network representation of protein structures: implications for protein stability.
Brinda et al. (2005)
11 more