› 1lvw

X-ray diffraction
1.7Å resolution

Crystal structure of glucose-1-phosphate thymidylyltransferase, RmlA, complex with dTDP

Released:
DOI: 10.2210/pdb1lvw/pdb
PDB entry 1lvw coloured by chain, front view.
PDB entry 1lvw coloured by chain, side view.
PDB entry 1lvw coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Entry authors: Dong A, Christendat D, Pai EF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glucose-1-phosphate thymidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 295 amino acids
Theoretical weight: 33.37 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O27819 (Residues: 1-292; Coverage: 100%)
Gene name: MTH_1791
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

4 bound ligands:
Ligand CL 5 x CL
Ligand SO4 2 x SO4
Ligand TYD 8 x TYD
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P212121
Unit cell:
a: 112.68Å b: 115.88Å c: 116.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.215
Expression system: Escherichia coli

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