› 1lwj

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF T. MARITIMA 4-ALPHA-GLUCANOTRANSFERASE/ACARBOSE COMPLEX

Released:
DOI: 10.2210/pdb1lwj/pdb
PDB entry 1lwj coloured by chain, front view.
PDB entry 1lwj coloured by chain, side view.
PDB entry 1lwj coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of Thermotoga maritima 4-alpha-glucanotransferase and its acarbose complex: implications for substrate specificity and catalysis.
Roujeinikova A, Raasch C, Sedelnikova S, Liebl W, Rice DW
J Mol Biol 321 149-62 (2002)
PMID: 12139940

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-alpha-glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 441 amino acids
Theoretical weight: 51.93 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: P80099 (Residues: 1-441; Coverage: 100%)
Gene names: TM_0364, mgtA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACG 2 x ACG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I222
Unit cell:
a: 94.542Å b: 181.354Å c: 197.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.302
Expression system: Escherichia coli

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Citations

4 review citations

Calcium binding proteins and calcium signaling in prokaryotes.
Domínguez et al. (2015)
3 more

3 mentions without citation

Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target.
Syson et al. (2011)
2 more