› 1lzs

X-ray diffraction
1.6Å resolution

STRUCTURAL CHANGES OF THE ACTIVE SITE CLEFT AND DIFFERENT SACCHARIDE BINDING MODES IN HUMAN LYSOZYME CO-CRYSTALLIZED WITH HEXA-N-ACETYL-CHITOHEXAOSE AT PH 4.0

Released:
DOI: 10.2210/pdb1lzs/pdb
PDB entry 1lzs coloured by chain, front view.
PDB entry 1lzs coloured by chain, side view.
PDB entry 1lzs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural changes of active site cleft and different saccharide binding modes in human lysozyme co-crystallized with hexa-N-acetyl-chitohexaose at pH 4.0.
Song H, Inaka K, Maenaka K, Matsushima M
J Mol Biol 244 522-40 (1994)
PMID: 7990138

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lysozyme C Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.72 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P61626 (Residues: 19-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 46.99Å b: 162.25Å c: 32.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Molecular divergence of lysozymes and alpha-lactalbumin.
Qasba et al. (1997)
1 more

8 mentions without citation

Enhanced antimicrobial activity of engineered human lysozyme.
Scanlon et al. (2010)
7 more