› 1m06

X-ray diffraction
3.5Å resolution

Structural Studies of Bacteriophage alpha3 Assembly, X-Ray Crystallography

Released:
DOI: 10.2210/pdb1m06/pdb
PDB entry 1m06 coloured by chain, front view.
PDB entry 1m06 coloured by chain, side view.
PDB entry 1m06 coloured by chain, top view.
Source organism: Escherichia phage alpha3
Primary publication:
Structural studies of bacteriophage alpha3 assembly.
Bernal RA, Hafenstein S, Olson NH, Bowman VD, Chipman PR, Baker TS, Fane BA, Rossmann MG
J Mol Biol 325 11-24 (2003)
PMID: 12473449

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
PDBe Complex ID:
PDB-CPX-114158 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (4 distinct):
Capsid protein F Chain: F
Molecule details ›
Chain: F
Length: 431 amino acids
Theoretical weight: 49.29 KDa
Source organism: Escherichia phage alpha3
Expression system: Escherichia coli
UniProt:
  • Canonical: P08767 (Residues: 1-431; Coverage: 100%)
Gene name: F
Sequence domains: Capsid protein (F protein)
Structure domains: Microviridae F protein
Major spike protein G Chain: G
Molecule details ›
Chain: G
Length: 187 amino acids
Theoretical weight: 19.6 KDa
Source organism: Escherichia phage alpha3
Expression system: Not provided
UniProt:
  • Canonical: P31281 (Residues: 1-187; Coverage: 100%)
Gene name: G
Sequence domains: Major spike protein (G protein)
Structure domains: Jelly Rolls
DNA-binding protein J Chain: J
Molecule details ›
Chain: J
Length: 24 amino acids
Theoretical weight: 2.83 KDa
Source organism: Escherichia phage alpha3
Expression system: Not provided
UniProt:
  • Canonical: P69548 (Residues: 1-24; Coverage: 100%)
Gene name: J
Sequence domains: Microvirus J protein
5'-D(P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR)P*(3DR))-3' Chain: X
Molecule details ›
Chain: X
Length: 10 nucleotides
Theoretical weight: 1.76 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 290.258Å b: 332.115Å c: 337.698Å
α: 90° β: 94.08° γ: 90°
R-values:
R R work R free
0.232 0.232 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Theoretical aspects of virus capsid assembly.
Zlotnick A. (2005)
5 more

8 mentions without citation

Diversity of environmental single-stranded DNA phages revealed by PCR amplification of the partial major capsid protein.
Hopkins et al. (2014)
7 more