› 1m0l

X-ray diffraction
1.47Å resolution

BACTERIORHODOPSIN/LIPID COMPLEX AT 1.47 A RESOLUTION

Released:
DOI: 10.2210/pdb1m0l/pdb
PDB entry 1m0l coloured by chain, front view.
PDB entry 1m0l coloured by chain, side view.
PDB entry 1m0l coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Crystallographic structure of the K intermediate of bacteriorhodopsin: conservation of free energy after photoisomerization of the retinal.
Schobert B, Cupp-Vickery J, Hornak V, Smith S, Lanyi J
J Mol Biol 321 715-26 (2002)
PMID: 12206785

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.27 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 1-262; Coverage: 100%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand LI1 13 x LI1
Ligand SQU 1 x SQU
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P63
Unit cell:
a: 60.94Å b: 60.94Å c: 110.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.149 0.179
Expression system: Halobacterium salinarum

Quick links

Citations

16 review citations

Bacteriorhodopsin.
Lanyi JK. (2004)
15 more

26 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
25 more