› 1m0u

X-ray diffraction
1.75Å resolution

Crystal Structure of the Drosophila Glutathione S-transferase-2 in Complex with Glutathione

Released:
DOI: 10.2210/pdb1m0u/pdb
PDB entry 1m0u coloured by chain, front view.
PDB entry 1m0u coloured by chain, side view.
PDB entry 1m0u coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of a Drosophila sigma class glutathione S-transferase reveals a novel active site topography suited for lipid peroxidation products.
Agianian B, Tucker PA, Schouten A, Leonard K, Bullard B, Gros P
J Mol Biol 326 151-65 (2003)
PMID: 12547198

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase S1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.64 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P41043 (Residues: 1-249; Coverage: 100%)
Gene names: CG8938, GST2, GSTS1-1, GstS1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 1 x GSH
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P3121
Unit cell:
a: 89.723Å b: 89.723Å c: 131.789Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.213 0.232
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The molecular basis of insecticide resistance in mosquitoes.
Hemingway et al. (2004)
5 more

4 mentions without citation

Glutathione transferases are structural and functional outliers in the thioredoxin fold.
Atkinson et al. (2009)
3 more