› 1m1f

X-ray diffraction
1.4Å resolution

Kid toxin protein from E.coli plasmid R1

Released:
DOI: 10.2210/pdb1m1f/pdb
PDB entry 1m1f coloured by chain, front view.
PDB entry 1m1f coloured by chain, side view.
PDB entry 1m1f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and functional analysis of the kid toxin protein from E. coli plasmid R1.
Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareño R, de la Cueva-Méndez G, Boelens R, Díaz-Orejas R, Rafferty JB
Structure 10 1425-33 (2002)
PMID: 12377128

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease PemK Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 11.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P13976 (Residues: 24-133; Coverage: 83%)
Gene name: pemK
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 33.264Å b: 45.457Å c: 65.021Å
α: 90° β: 96.12° γ: 90°
R-values:
R R work R free
0.161 0.16 0.182
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Prokaryotic toxin-antitoxin stress response loci.
Gerdes et al. (2005)
13 more

13 mentions without citation

Structure, Evolution, and Functions of Bacterial Type III Toxin-Antitoxin Systems.
Goeders et al. (2016)
12 more