› 1m1g

X-ray diffraction
2Å resolution

Crystal Structure of Aquifex aeolicus N-utilization substance G (NusG), Space Group P2(1)

Released:
DOI: 10.2210/pdb1m1g/pdb
PDB entry 1m1g coloured by chain, front view.
PDB entry 1m1g coloured by chain, side view.
PDB entry 1m1g coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structures of transcription factor NusG in light of its nucleic acid- and protein-binding activities.
Steiner T, Kaiser JT, Marinkoviç S, Huber R, Wahl MC
EMBO J 21 4641-53 (2002)
PMID: 12198166

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination/antitermination protein NusG Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 248 amino acids
Theoretical weight: 28.04 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67757 (Residues: 1-248; Coverage: 100%)
Gene names: aq_1931, nusG
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 87.5Å b: 54.4Å c: 113.2Å
α: 90° β: 89.3° γ: 90°
R-values:
R R work R free
0.251 0.251 0.274
Expression system: Escherichia coli

Quick links

Citations

13 review citations

A nexus for gene expression-molecular mechanisms of Spt5 and NusG in the three domains of life.
Werner F. (2012)
12 more

11 mentions without citation

Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.
Martinez-Rucobo et al. (2011)
10 more