› 1m2d

X-ray diffraction
1.05Å resolution

Crystal structure at 1.05 Angstroms resolution of the Cys59Ser variant of the thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus

Released:
DOI: 10.2210/pdb1m2d/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1m2d coloured by chain, front view.
PDB entry 1m2d coloured by chain, side view.
PDB entry 1m2d coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
High resolution crystal structures of the wild type and Cys-55-->Ser and Cys-59-->Ser variants of the thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus.
Yeh AP, Ambroggio XI, Andrade SL, Einsle O, Chatelet C, Meyer J, Rees DC
J Biol Chem 277 34499-507 (2002)
PMID: 12089152

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin, 2Fe-2S Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.19 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66511 (Residues: 2-111; Coverage: 99%)
Gene names: aq_107, aq_108A, fdx4
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 67.3Å b: 59.8Å c: 46.8Å
α: 90° β: 109.3° γ: 90°
R-values:
R R work R free
0.138 0.137 0.162
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Iron-sulfur protein folds, iron-sulfur chemistry, and evolution.
Meyer J. (2008)
2 more

2 mentions without citation

Evolutionary Relationships Between Low Potential Ferredoxin and Flavodoxin Electron Carriers.
Campbell et al. (2019)
1 more