› 1m2n

X-ray diffraction
2.6Å resolution

Sir2 homologues (D102G/F159A/R170A) mutant-2'-O-acetyl ADP ribose complex

Released:
DOI: 10.2210/pdb1m2n/pdb
PDB entry 1m2n coloured by chain, front view.
PDB entry 1m2n coloured by chain, side view.
PDB entry 1m2n coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural basis for the NAD-dependent deacetylase mechanism of Sir2.
Chang JH, Kim HC, Hwang KY, Lee JW, Jackson SP, Bell SD, Cho Y
J Biol Chem 277 34489-98 (2002)
PMID: 12091395

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.43 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28597 (Residues: 1-245; Coverage: 100%)
Gene names: AF_1676, cobB1
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand OAD 2 x OAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 84.72Å b: 84.72Å c: 193.087Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.308
Expression system: Escherichia coli

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Citations

25 review citations

The biochemistry of sirtuins.
Sauve et al. (2006)
24 more