› 1m2t

X-ray diffraction
1.89Å resolution

Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution

Released:
DOI: 10.2210/pdb1m2t/pdb
PDB entry 1m2t coloured by chain, front view.
PDB entry 1m2t coloured by chain, side view.
PDB entry 1m2t coloured by chain, top view.
Source organism: Viscum album
Primary publication:
Crystallisation under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9 A resolution.
Krauspenhaar R, Rypniewski W, Kalkura N, Moore K, DeLucas L, Stoeva S, Mikhailov A, Voelter W, Betzel Ch
Acta Crystallogr D Biol Crystallogr 58 1704-7 (2002)
PMID: 12351890

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-galactoside-specific lectin 1 chain A isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.82 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 34-287; Coverage: 48%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Beta-galactoside-specific lectin 1 chain B Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 28.57 KDa
Source organism: Viscum album
UniProt:
  • Canonical: P81446 (Residues: 302-510, 512-563; Coverage: 49%)
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

4 bound ligands:
Ligand NAG 6 x NAG
Ligand FUC 2 x FUC
Ligand ADE 1 x ADE
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P6522
Unit cell:
a: 107.12Å b: 107.12Å c: 309.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.24

Quick links

Citations

6 citation in other articles

Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose.
Niwa et al. (2003)
5 more

4 mentions without citation

Analysis on conservation of disulphide bonds and their structural features in homologous protein domain families.
Thangudu et al. (2008)
3 more