› 1m3j

X-ray diffraction
3Å resolution

CRYSTAL form II of perfringolysin O

Released:
DOI: 10.2210/pdb1m3j/pdb
PDB entry 1m3j coloured by chain, front view.
PDB entry 1m3j coloured by chain, side view.
PDB entry 1m3j coloured by chain, top view.
Source organism: Clostridium perfringens
Entry authors: Rossjohn J, Parker M, Polekhina G, Feil S, Tweten R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Perfringolysin O Chains: A, B
Molecule details ›
Chains: A, B
Length: 471 amino acids
Theoretical weight: 52.57 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2E9 (Residues: 30-500; Coverage: 100%)
Gene names: CPE0163, pfo, pfoA, pfoR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21212
Unit cell:
a: 167Å b: 214.11Å c: 47.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.337
Expression system: Escherichia coli

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