› 1m4d

X-ray diffraction
1.8Å resolution

Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis-Complex with Coenzyme A and Tobramycin

Released:
DOI: 10.2210/pdb1m4d/pdb
PDB entry 1m4d coloured by chain, front view.
PDB entry 1m4d coloured by chain, side view.
PDB entry 1m4d coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates.
Vetting MW, Hegde SS, Javid-Majd F, Blanchard JS, Roderick SL
Nat Struct Biol 9 653-8 (2002)
PMID: 12161746

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162076 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 2'-N-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 20.06 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQG9 (Residues: 1-181; Coverage: 100%)
Gene names: MTCY06A4.06c, Rv0262c, aac
Sequence domains: Acetyltransferase (GNAT) domain
Structure domains: Aminopeptidase

Ligands and Environments

3 bound ligands:
Ligand TOY 2 x TOY
Ligand COA 2 x COA
Ligand PAP 1 x PAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 48.7Å b: 86.6Å c: 98.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.174 0.211
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structure and functions of the GNAT superfamily of acetyltransferases.
Vetting et al. (2005)
16 more

7 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
6 more