› 1m64

X-ray diffraction
1.8Å resolution

Crystal structure of Q363F mutant flavocytochrome c3

Released:
DOI: 10.2210/pdb1m64/pdb
PDB entry 1m64 coloured by chain, front view.
PDB entry 1m64 coloured by chain, side view.
PDB entry 1m64 coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
Engineering water to act as an active site acid catalyst in a soluble fumarate reductase.
Mowat CG, Pankhurst KL, Miles CS, Leys D, Walkinshaw MD, Reid GA, Chapman SK
Biochemistry 41 11990-6 (2002)
PMID: 12356299

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate reductase (cytochrome) Chains: A, B
Molecule details ›
Chains: A, B
Length: 571 amino acids
Theoretical weight: 60.65 KDa
Source organism: Shewanella frigidimarina
Expression system: Shewanella frigidimarina
UniProt:
  • Canonical: P0C278 (Residues: 1-571; Coverage: 100%)
Gene names: fcc3, fccA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 8 x HEM

Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand NA 2 x NA
Ligand FUM 2 x FUM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 78.52Å b: 88.886Å c: 91.194Å
α: 90° β: 104.42° γ: 90°
R-values:
R R work R free
0.163 0.163 0.224
Expression system: Shewanella frigidimarina

Quick links

Citations

4 review citations

Function and structure of complex II of the respiratory chain.
Cecchini G. (2003)
3 more