› 1m6t

X-ray diffraction
1.81Å resolution

CRYSTAL STRUCTURE OF B562RIL, A REDESIGNED FOUR HELIX BUNDLE

Released:
DOI: 10.2210/pdb1m6t/pdb
PDB entry 1m6t coloured by chain, front view.
PDB entry 1m6t coloured by chain, side view.
PDB entry 1m6t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Redesign of a four-helix bundle protein by phage display coupled with proteolysis and structural characterization by NMR and X-ray crystallography.
Chu R, Takei J, Knowlton JR, Andrykovitch M, Pei W, Kajava AV, Steinbach PJ, Ji X, Bai Y
J Mol Biol 323 253-62 (2002)
PMID: 12381319

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2221
Unit cell:
a: 41.3Å b: 52.34Å c: 93.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

A new generation of protein display scaffolds for molecular recognition.
Hosse et al. (2006)
8 more

36 mentions without citation

Protein scaffold-based molecular probes for cancer molecular imaging.
Miao et al. (2011)
35 more