› 1m7h

X-ray diffraction
2Å resolution

Crystal Structure of APS kinase from Penicillium Chrysogenum: Structure with APS soaked out of one dimer

Released:
DOI: 10.2210/pdb1m7h/pdb
PDB entry 1m7h coloured by chain, front view.
PDB entry 1m7h coloured by chain, side view.
PDB entry 1m7h coloured by chain, top view.
Source organism: Penicillium chrysogenum
Primary publication:
Ligand-induced structural changes in adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum.
Lansdon EB, Segel IH, Fisher AJ
Biochemistry 41 13672-80 (2002)
PMID: 12427029

Function and Biology Details

Reaction catalysed: 
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-171380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 23.8 KDa
Source organism: Penicillium chrysogenum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q12657 (Residues: 1-211; Coverage: 100%)
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 12 x SO4
Ligand ADP 4 x ADP
Ligand ADX 2 x ADX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 83.601Å b: 83.688Å c: 138.677Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.258
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

The power of vanadate in crystallographic investigations of phosphoryl transfer enzymes.
Davies et al. (2004)
1 more

3 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
2 more