› 1m8t

X-ray diffraction
2.1Å resolution

Structure of an acidic Phospholipase A2 from the venom of Ophiophagus hannah at 2.1 resolution from a hemihedrally twinned crystal form

Released:
DOI: 10.2210/pdb1m8t/pdb
PDB entry 1m8t coloured by chain, front view.
PDB entry 1m8t coloured by chain, side view.
PDB entry 1m8t coloured by chain, top view.
Source organism: Ophiophagus hannah
Primary publication:
Structure of a king cobra phospholipase A2 determined from a hemihedrally twinned crystal.
Xu S, Gu L, Wang Q, Shu Y, Song S, Lin Z
Acta Crystallogr D Biol Crystallogr 59 1574-81 (2003)
PMID: 12925787

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acidic phospholipase A2 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 119 amino acids
Theoretical weight: 13.2 KDa
Source organism: Ophiophagus hannah
UniProt:
  • Canonical: Q9DF33 (Residues: 28-146; Coverage: 95%)
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand HEZ 10 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P63
Unit cell:
a: 98.06Å b: 98.06Å c: 132.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.192 0.213

Quick links

Citations

1 review citation

Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis.
Kang et al. (2011)
3 other articles

1 mention without citation

Crystal structure of a class XIB phospholipase A2 (PLA2): rice (oryza sativa) isoform-2 pla2 and an octanoate complex.
Guy et al. (2009)