› 1m9g

Solution NMR

Solution structure of G16A-MNEI, a structural mutant of single chain monellin MNEI

Released:
DOI: 10.2210/pdb1m9g/pdb
PDB entry 1m9g coloured by chain, ensemble of 20 models, front view.
PDB entry 1m9g coloured by chain, ensemble of 20 models, side view.
PDB entry 1m9g coloured by chain, ensemble of 20 models, top view.
Source organism: Dioscoreophyllum cumminsii
Primary publication:
The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI.
Spadaccini R, Trabucco F, Saviano G, Picone D, Crescenzi O, Tancredi T, Temussi PA
J Mol Biol 328 683-92 (2003)
PMID: 12706725

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monellin chain B Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.43 KDa
Source organism: Dioscoreophyllum cumminsii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02882 (Residues: 1-48; Coverage: 96%)
Sequence domains: Monellin
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics and restrained energy minimization
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Developments in biotechnological production of sweet proteins.
Masuda et al. (2006)
2 more

2 mentions without citation

Toward the understanding of MNEI sweetness from hydration map surfaces.
De Simone et al. (2006)
1 more