› 1m9n

X-ray diffraction
1.93Å resolution

CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH AICAR AND XMP AT 1.93 ANGSTROMS.

Released:
DOI: 10.2210/pdb1m9n/pdb
PDB entry 1m9n coloured by chain, front view.
PDB entry 1m9n coloured by chain, side view.
PDB entry 1m9n coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural insights into the avian AICAR transformylase mechanism.
Wolan DW, Greasley SE, Beardsley GP, Wilson IA
Biochemistry 41 15505-13 (2002)
PMID: 12501179

Function and Biology Details

Reactions catalysed: 
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional purine biosynthesis protein ATIC Chains: A, B
Molecule details ›
Chains: A, B
Length: 613 amino acids
Theoretical weight: 66.67 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31335 (Residues: 1-593; Coverage: 100%)
Gene names: ATIC, PURH
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand K 2 x K
Ligand AMZ 2 x AMZ
Ligand XMP 2 x XMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 56.48Å b: 107.88Å c: 103.86Å
α: 90° β: 91.2° γ: 90°
R-values:
R R work R free
0.21 0.206 0.244
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
12 other articles

5 mentions without citation

Identification of the Formycin A Biosynthetic Gene Cluster from Streptomyces kaniharaensis Illustrates the Interplay between Biological Pyrazolopyrimidine Formation and de Novo Purine Biosynthesis.
Wang et al. (2019)
4 more