› 1mby

X-ray diffraction
2Å resolution

Murine Sak Polo Domain

Released:
DOI: 10.2210/pdb1mby/pdb
PDB entry 1mby coloured by chain, front view.
PDB entry 1mby coloured by chain, side view.
PDB entry 1mby coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization.
Leung GC, Hudson JW, Kozarova A, Davidson A, Dennis JW, Sicheri F
Nat Struct Biol 9 719-24 (2002)
PMID: 12352953

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 88 amino acids
Theoretical weight: 9.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64702 (Residues: 839-925; Coverage: 9%)
Gene names: Plk4, Sak, Stk18
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3212
Unit cell:
a: 51.782Å b: 51.782Å c: 146.941Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.249
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Polo-like kinases and the orchestration of cell division.
Barr et al. (2004)
32 more

3 mentions without citation

Building a ninefold symmetrical barrel: structural dissections of centriole assembly.
Dong G. (2015)
2 more