› 1mdq

X-ray diffraction
1.9Å resolution

REFINED STRUCTURES OF TWO INSERTION(SLASH)DELETION MUTANTS PROBE FUNCTION OF THE MALTODEXTRIN BINDING PROTEIN

Released:
DOI: 10.2210/pdb1mdq/pdb
PDB entry 1mdq coloured by chain, front view.
PDB entry 1mdq coloured by chain, side view.
PDB entry 1mdq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Refined structures of two insertion/deletion mutants probe function of the maltodextrin binding protein.
Sharff AJ, Rodseth LE, Szmelcman S, Hofnung M, Quiocho FA
J Mol Biol 246 8-13 (1995)
PMID: 7853407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 40.83 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0AEX9 (Residues: 27-325, 326-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 106.01Å b: 66.46Å c: 57.75Å
α: 90° β: 113.17° γ: 90°
R-values:
R R work R free
0.22 0.22 not available
Expression system: Not provided

Quick links

Citations

1 review citation

Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.
Boos et al. (1998)
10 other articles

1 mention without citation

Development of quantitative structure-binding affinity relationship models based on novel geometrical chemical descriptors of the protein-ligand interfaces.
Zhang et al. (2006)