› 1mey

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA

Released:
DOI: 10.2210/pdb1mey/pdb
PDB entry 1mey coloured by chain, front view.
PDB entry 1mey coloured by chain, side view.
PDB entry 1mey coloured by chain, top view.
Source organisms:
Primary publication:
A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA.
Kim CA, Berg JM
Nat Struct Biol 3 940-5 (1996)
PMID: 8901872

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-114179 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CONSENSUS ZINC FINGER Chains: C, F, G
Molecule details ›
Chains: C, F, G
Length: 87 amino acids
Theoretical weight: 10.15 KDa
Source organism: Unidentified
Expression system: Escherichia coli BL21(DE3)
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*TP*GP*AP*GP*GP*CP*AP*GP*AP*AP*CP*T)-3') Chains: A, D
Molecule details ›
Chains: A, D
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*AP*GP*TP*TP*CP*TP*GP*CP*CP*TP*(C38)P*A)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 13 nucleotides
Theoretical weight: 4.04 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand CL 1 x CL
1 modified residue:
Ligand C38 2 x C38

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 62.07Å b: 165.53Å c: 46.274Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.319
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Sp1- and Krüppel-like transcription factors.
Kaczynski et al. (2003)
13 more

40 mentions without citation

Genome engineering with zinc-finger nucleases.
Carroll D. (2011)
39 more