› 1mfa

X-ray diffraction
1.7Å resolution

STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1mfa/pdb
PDB entry 1mfa coloured by chain, front view.
PDB entry 1mfa coloured by chain, side view.
PDB entry 1mfa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution.
Zdanov A, Li Y, Bundle DR, Deng SJ, MacKenzie CR, Narang SA, Young NM, Cygler M
Proc Natl Acad Sci U S A 91 6423-7 (1994)
PMID: 7517550

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213041 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
IGG1-LAMBDA SE155-4 FAB (LIGHT CHAIN) Chain: L
Molecule details ›
Chain: L
Length: 127 amino acids
Theoretical weight: 13.31 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
IGG1-LAMBDA SE155-4 FAB (HEAVY CHAIN) Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.2 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: MMA, GLA, ABE
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 53.1Å b: 61Å c: 74.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.166 not available
Expression system: Not provided

Quick links

Citations

8 review citations

The impact of glycosylation on the biological function and structure of human immunoglobulins.
Arnold et al. (2007)
7 more

23 mentions without citation

Macromolecular modeling and design in Rosetta: recent methods and frameworks.
Leman et al. (2020)
22 more