› 1mfi

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF MACROPHAGE MIGRATION INHIBITORY FACTOR COMPLEXED WITH (E)-2-FLUORO-P-HYDROXYCINNAMATE

Released:
DOI: 10.2210/pdb1mfi/pdb
PDB entry 1mfi coloured by chain, front view.
PDB entry 1mfi coloured by chain, side view.
PDB entry 1mfi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of macrophage migration inhibitory factor complexed with (E)-2-fluoro-p-hydroxycinnamate at 1.8 A resolution: implications for enzymatic catalysis and inhibition.
Taylor AB, Johnson WH, Czerwinski RM, Li HS, Hackert ML, Whitman CP
Biochemistry 38 7444-52 (1999)
PMID: 10360941

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P34884 (Residues: 2-115; Coverage: 99%)
Gene name: Mif
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand FHC 3 x FHC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 99.53Å b: 99.53Å c: 105.69Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.178 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

MIF: a new cytokine link between rheumatoid arthritis and atherosclerosis.
Morand et al. (2006)
2 more

4 mentions without citation

Blockade of MIF-CD74 Signalling on Macrophages and Dendritic Cells Restores the Antitumour Immune Response Against Metastatic Melanoma.
Figueiredo et al. (2018)
3 more