› 1mfq

X-ray diffraction
3.1Å resolution

Crystal Structure Analysis of a Ternary S-Domain Complex of Human Signal Recognition Particle

Released:
DOI: 10.2210/pdb1mfq/pdb
PDB entry 1mfq coloured by chain, front view.
PDB entry 1mfq coloured by chain, side view.
PDB entry 1mfq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Induced structural changes of 7SL RNA during the assembly of human signal recognition particle.
Kuglstatter A, Oubridge C, Nagai K
Nat Struct Biol 9 740-4 (2002)
PMID: 12244299

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140556 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 19 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09132 (Residues: 14-120; Coverage: 74%)
Gene name: SRP19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
Signal recognition particle subunit SRP54 Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61011 (Residues: 323-441; Coverage: 24%)
Gene name: SRP54
Sequence domains: Signal peptide binding domain
Structure domains: Signal recognition particle, SRP54 subunit, M-domain
7S RNA of human SRP Chain: A
Molecule details ›
Chain: A
Length: 128 nucleotides
Theoretical weight: 41.57 KDa
Sequence domains: Metazoan signal recognition particle RNA

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand CL 2 x CL
1 modified residue:
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6522
Unit cell:
a: 131.18Å b: 131.18Å c: 204.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.267
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Analysis of RNA motifs.
Leontis et al. (2003)
10 more

26 mentions without citation

Structure, function and evolution of the signal recognition particle.
Nagai et al. (2003)
25 more