› 1mfw

X-ray diffraction
1.6Å resolution

STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: SELENOMETHIONINE LABELED PROTEIN

Released:
DOI: 10.2210/pdb1mfw/pdb
PDB entry 1mfw coloured by chain, front view.
PDB entry 1mfw coloured by chain, side view.
PDB entry 1mfw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The DCX-domain tandems of doublecortin and doublecortin-like kinase.
Kim MH, Cierpicki T, Derewenda U, Krowarsch D, Feng Y, Devedjiev Y, Dauter Z, Walsh CA, Otlewski J, Bushweller JH, Derewenda ZS
Nat Struct Biol 10 324-33 (2003)
PMID: 12692530

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase DCLK1 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15075 (Residues: 49-154; Coverage: 14%)
Gene names: DCAMKL1, DCDC3A, DCLK1, KIAA0369
Sequence domains: Doublecortin
Structure domains: Doublecortin domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 38.785Å b: 29.43Å c: 40.099Å
α: 90° β: 115.72° γ: 90°
R-values:
R R work R free
0.132 0.127 0.18
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Uses for JNK: the many and varied substrates of the c-Jun N-terminal kinases.
Bogoyevitch et al. (2006)
15 more

1 mention without citation

Stretching to understand proteins - a survey of the protein data bank.
SuĊ‚kowska et al. (2008)