› 1mgo

X-ray diffraction
1.2Å resolution

Horse Liver Alcohol Dehydrogenase Phe93Ala Mutant

Released:
DOI: 10.2210/pdb1mgo/pdb
PDB entry 1mgo coloured by chain, front view.
PDB entry 1mgo coloured by chain, side view.
PDB entry 1mgo coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Mobility of fluorobenzyl alcohols bound to liver alcohol dehydrogenases as determined by NMR and X-ray crystallographic studies.
Rubach JK, Plapp BV
Biochemistry 41 15770-9 (2002)
PMID: 12501206

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.78 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand ZN 4 x ZN
Ligand PFB 2 x PFB
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 44.144Å b: 51.308Å c: 93.481Å
α: 91.98° β: 103.01° γ: 109.81°
R-values:
R R work R free
0.188 0.188 0.209
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Relating protein motion to catalysis.
Hammes-Schiffer et al. (2006)
5 other articles

5 mentions without citation

PharmMapper server: a web server for potential drug target identification using pharmacophore mapping approach.
Liu et al. (2010)
4 more