› 1mgw

X-ray diffraction
2Å resolution

Crystal structure of RNase Sa3, cytotoxic microbial ribonuclease

Released:
DOI: 10.2210/pdb1mgw/pdb
PDB entry 1mgw coloured by chain, front view.
PDB entry 1mgw coloured by chain, side view.
PDB entry 1mgw coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Primary publication:
X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity.
Sevcik J, Urbanikova L, Leland PA, Raines RT
J Biol Chem 277 47325-30 (2002)
PMID: 12228255

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa3 Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 11.06 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30289 (Residues: 43-141; Coverage: 94%)
Gene name: rnaSA3
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand LI 1 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P3121
Unit cell:
a: 64.72Å b: 64.72Å c: 69.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.212
Expression system: Escherichia coli

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Citations

7 review citations

Cytotoxic ribonucleases: molecular weapons and their targets.
Makarov et al. (2003)
6 more

2 mentions without citation

Contribution of single tryptophan residues to the fluorescence and stability of ribonuclease Sa.
Alston et al. (2004)
1 more