› 1mhh

X-ray diffraction
2.1Å resolution

Structure of P. magnus protein L mutant bound to a mouse Fab

Released:
DOI: 10.2210/pdb1mhh/pdb
PDB entry 1mhh coloured by chain, front view.
PDB entry 1mhh coloured by chain, side view.
PDB entry 1mhh coloured by chain, top view.
Source organisms:
Primary publication:
Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface.
Graille M, Harrison S, Crump MP, Findlow SC, Housden NG, Muller BH, Battail-Poirot N, Sibaï G, Sutton BJ, Taussig MJ, Jolivet-Reynaud C, Gore MG, Stura EA
J Biol Chem 277 47500-6 (2002)
PMID: 12221088

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211571 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab, light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 220 amino acids
Theoretical weight: 24.39 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab, heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 217 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
protein L domain C Chains: E, F
Molecule details ›
Chains: E, F
Length: 63 amino acids
Theoretical weight: 6.92 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
1 modified residue:
Ligand AEA 2 x AEA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 78.486Å b: 100.957Å c: 149.147Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Screening isolates from antibody phage-display libraries.
Buckler et al. (2008)
2 more

6 mentions without citation

Antibody-protein interactions: benchmark datasets and prediction tools evaluation.
Ponomarenko et al. (2007)
5 more