› 1mhp

X-ray diffraction
2.8Å resolution

Crystal structure of a chimeric alpha1 integrin I-domain in complex with the Fab fragment of a humanized neutralizing antibody

Released:
DOI: 10.2210/pdb1mhp/pdb
PDB entry 1mhp coloured by chain, front view.
PDB entry 1mhp coloured by chain, side view.
PDB entry 1mhp coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the alpha1beta1 integrin I domain in complex with an antibody Fab fragment.
Karpusas M, Ferrant J, Weinreb PH, Carmillo A, Taylor FR, Garber EA
J Mol Biol 327 1031-41 (2003)
PMID: 12662928

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210743 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Integrin alpha-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.63 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18614 (Residues: 169-360; Coverage: 17%)
Gene name: Itga1
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Fab fragment, heavy chain Chains: H, X
Molecule details ›
Chains: H, X
Length: 219 amino acids
Theoretical weight: 23.14 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAB FRAGMENT, light chain Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 212 amino acids
Theoretical weight: 23.2 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P65
Unit cell:
a: 255.09Å b: 255.09Å c: 38.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.213 0.272
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

4 review citations

Integrin structure, allostery, and bidirectional signaling.
Arnaout et al. (2005)
3 more

11 mentions without citation

Computer-aided antibody design.
Kuroda et al. (2012)
10 more