› 1mi7

X-ray diffraction
2.5Å resolution

Crystal Structure of Domain Swapped trp Aporepressor in 30%(v/v) Isopropanol

Released:
DOI: 10.2210/pdb1mi7/pdb
PDB entry 1mi7 coloured by chain, front view.
PDB entry 1mi7 coloured by chain, side view.
PDB entry 1mi7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
E. coli trp repressor forms a domain-swapped array in aqueous alcohol.
Lawson CL, Benoff B, Berger T, Berman HM, Carey J
Structure 12 1099-108 (2004)
PMID: 15274929

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trp operon repressor Chain: R
Molecule details ›
Chain: R
Length: 107 amino acids
Theoretical weight: 12.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A881 (Residues: 2-108; Coverage: 99%)
Gene names: JW4356, b4393, rtrY, trpR
Sequence domains: Trp repressor protein
Structure domains: TrpR-like

Ligands and Environments

1 bound ligand:
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6122
Unit cell:
a: 85.31Å b: 85.31Å c: 113.968Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.253 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Deposition diseases and 3D domain swapping.
Bennett et al. (2006)
2 more

10 mentions without citation

Linking in domain-swapped protein dimers.
Baiesi et al. (2016)
9 more