› 1mif

X-ray diffraction
2.6Å resolution

MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)

Released:
DOI: 10.2210/pdb1mif/pdb
PDB entry 1mif coloured by chain, front view.
PDB entry 1mif coloured by chain, side view.
PDB entry 1mif coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor.
Sun HW, Bernhagen J, Bucala R, Lolis E
Proc Natl Acad Sci U S A 93 5191-6 (1996)
PMID: 8643551

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 115 amino acids
Theoretical weight: 12.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14174 (Residues: 1-115; Coverage: 100%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 96.8Å b: 96.8Å c: 106.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

46 review citations

Macrophage migration inhibitory factor: a regulator of innate immunity.
Calandra et al. (2003)
45 more

17 mentions without citation

D-dopachrome tautomerase (D-DT or MIF-2): doubling the MIF cytokine family.
Merk et al. (2012)
16 more