› 1mj2

X-ray diffraction
2.4Å resolution

METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO A CONSENSUS OPERATOR SEQUENCE

Released:
DOI: 10.2210/pdb1mj2/pdb
PDB entry 1mj2 coloured by chain, front view.
PDB entry 1mj2 coloured by chain, side view.
PDB entry 1mj2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Direct and indirect readout in mutant Met repressor-operator complexes.
Garvie CW, Phillips SE
Structure 8 905-14 (2000)
PMID: 10986458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114183 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Met repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 104 amino acids
Theoretical weight: 12.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8U6 (Residues: 2-105; Coverage: 99%)
Gene names: JW3909, b3938, metJ
Sequence domains: Met Apo-repressor, MetJ
Structure domains: MET Apo-Repressor, subunit A
DNA (5'-D(*TP*TP*AP*GP*AP*CP*GP*TP*CP*TP*AP*GP*AP*CP*GP*TP*CP*TP*A)-3') Chains: F, G
Molecule details ›
Chains: F, G
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SAM 4 x SAM
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P3221
Unit cell:
a: 117.18Å b: 117.18Å c: 90.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.26
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Protein flexibility in the light of structural alphabets.
Craveur et al. (2015)
17 other articles

9 mentions without citation

Protein-DNA binding specificity predictions with structural models.
Morozov et al. (2005)
8 more