› 1mjk

X-ray diffraction
2.15Å resolution

METHIONINE REPRESSOR MUTANT APOREPRESSOR (Q44K) FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1mjk/pdb
PDB entry 1mjk coloured by chain, front view.
PDB entry 1mjk coloured by chain, side view.
PDB entry 1mjk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystallographic studies of the methionine repressor-operator complex and the oc31 42kDa repressor
Garvie CW
Thesis, University of Leeds (1997)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Met repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 12.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8U6 (Residues: 2-105; Coverage: 99%)
Gene names: JW3909, b3938, metJ
Sequence domains: Met Apo-repressor, MetJ
Structure domains: MET Apo-Repressor, subunit A

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 35.68Å b: 63.57Å c: 44.61Å
α: 90° β: 102.55° γ: 90°
R-values:
R R work R free
0.181 0.181 0.223
Expression system: Escherichia coli

Quick links

Citations

2 mentions without citation

A protein-DNA docking benchmark.
van Dijk et al. (2008)
1 more