› 1mjq

X-ray diffraction
2.4Å resolution

METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO AN ALTERED MET CONSENSUS OPERATOR SEQUENCE

Released:
DOI: 10.2210/pdb1mjq/pdb
PDB entry 1mjq coloured by chain, front view.
PDB entry 1mjq coloured by chain, side view.
PDB entry 1mjq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Direct and indirect readout in mutant Met repressor-operator complexes.
Garvie CW, Phillips SE
Structure 8 905-14 (2000)
PMID: 10986458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114188 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Met repressor Chains: A, B, C, D, G, H, I, J
Molecule details ›
Chains: A, B, C, D, G, H, I, J
Length: 104 amino acids
Theoretical weight: 12.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8U6 (Residues: 2-105; Coverage: 99%)
Gene names: JW3909, b3938, metJ
Sequence domains: Met Apo-repressor, MetJ
Structure domains: MET Apo-Repressor, subunit A
MUTATED MET CONSENSUS OPERATOR DUPLEX Chains: E, F, K, L
Molecule details ›
Chains: E, F, K, L
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SAM 8 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P32
Unit cell:
a: 119.42Å b: 119.42Å c: 84.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 citation in other articles

DBD-Hunter: a knowledge-based method for the prediction of DNA-protein interactions.
Gao et al. (2008)
17 more

4 mentions without citation

Protein flexibility in the light of structural alphabets.
Craveur et al. (2015)
3 more